Mudter Professor of Cancer Research

Glycans that serve as cancer markers

Glycosyltransferase regulation during oncogenesis and differentiation

Lectins involved in innate immunity

Development of novel reagents that can recognize glycans (Glycan Recognition Reagents or GRR)

E-mail: hawkeye@uga.edu
Telephone: 706-542-1702
Fax: 706-542-4412

Publications (NCBI)

Dr. Pierce received his Ph.D. in biology in 1980 from the Johns Hopkins University. Prior to joining the CCRC in April 1991, Dr. Pierce was assistant then associate professor in the Department of Cell Biology and Anatomy at the University of Miami School of Medicine and Staff Investigator of the Papanicolau Comprehensive Cancer Center. In 1988 he was the recipient of a five-year Faculty Research Award from the American Cancer Society. He now serves on the editorial boards of the Journal of Biological Chemistry and Glycobiology. He now serves as Co-Chair of the Alliance for Glycobiologists for Cancer Detection, the Cancer Biology Chair of the Consortium for Functional Glycomics, and 2015 President of the Society for Glycobiology. He is P.I. of the NIGMS National Center for Biomedical Glycomics and Co-P.I. of the NIH Glycoscience Predoctoral Training Program (GTP). His funding comes from NIGMS and NCI.

To Top

• What are the precise glycan structural changes that occur?
• How these changes are regulated at the molecular level?
• What are the functional consequences of these changes?
• Can these changes be exploited to develop potential diagnostics or therapeutics?
• We isolated a cDNA encoding a glycosyltransferase whose activity and product is increased in many cancers, particularly epithelial-derived and showed that its transcript was upregulated via the ras-raf-ets oncogenic signaling pathway.
• Our main focus now is on a unique N-glycan whose expression is only on a few proteins in the human genome (protein-site-specific) and appears to be an excellent marker for pancreatic carcinoma.

Discovery of a novel family of innate immune lectins

• The X-lectin family is found in deuterostomes from the sea cucumber to man
• Over 240 sequences from lectins in this family are now in the database
• We isolated the Xenopus laevis cortical granule lectin and with Kelley Moremen showed that it had two human homologs
• These human homologs, the intelectins, are constitutively expressed in various endothelial cells, Paneth cells, and are induced in respiratory and intestinal epithelia by IL-13, an innate immune cytokine produced by immune cells
• Recent results show intelectin-1 binds and kills specific pathogenic bacteria
• We are defining the specificities of the binding sites on various intelectins and working to discover their structures and functions

Here is a recent seminar that I presented as part of the Goldstein Lectureship, University of Michigan Department of Biochemistry and Molecular Biology https://www.dropbox.com/s/pwt0tg02qjul49e/AnnArbor2014.pptx?dl=0

To Top

Journal Articles
Book Chapters are listed at the bottom of this page.

Y Zheng, A Pierce, WL Wagner, HV Scheller, D Mohnen, A Tsuda, M Ackermann, SJ Mentzer. 2020. Analysis of pectin biopolymer phase states using acoustic emissions. Carbohydrate Polymers Jan 1; 227: 115282-doi:10.1016. PMID:31590860

A Pierce, Y Zheng, WL Wagner, HV Scheller, D Mohnen, A Tsuda, M Ackermann, SJ Mentzer. 2020. Pectin biopolymer mechanics and microstructure associated with polysaccharide phase transitions. J Biomed Mater Res Part A 108(2): doi: 10.1002-jbm.a.36811. PMID:31595695

C Byun, Y Zheng, A Pierce, WL Wagner, HV Scheller, D Mohnen, M Ackermann, SJ Mentzer. 2020. Physical Properties of Low-Methoxyl Pectin Biopolymers. Molecules 25:75: -. PMID:31878302

A Pierce, Y Zheng, WL Wagner, HV Scheller, D Mohnen, M Ackermann, SJ Mentzer. 2020. Visualizing Pectin Polymer-Polymer Entanglement Produced by Interfacial Water Movement. Carbohydr Polymers Oct 15;246:116618: doi: 10.1016/-.carbpol.2020.11. PMID:32747258

Y Zheng, A Pierce, WL Wagner, HV Scheller, D Mohnen, M Ackermann, SJ Mentzer. 2018. Water-Dependent Blending of Pectin Films: The Mechanics of Conjoined Biopolymers. Molecules 25(9): doi: 10.3390-. PMID:32365966

M. Tiemeyer, K. Aoki, J. Paulson, R.D. Cummings, W.S. York, N.G. Karlsson, F. Lisacek, N.H. Packer, M.P. Campbell, N.P. Aoki, A. Fujita, M. Matsubara, D. Shinmachi, S. Tsuchiya, I. Yamada, M. Pierce, R. Ranzinger, H. Narimatsu, K.F. Aoki-Kinoshita. 2017. GlyTouCan: An accessible glycan structure repository. Glycobiology 27: 915-919. PMID:28922742

H Guo, B Zhang, A Nairn, T Nagy, KW Moremen, P Bukhaults, M Pierce. 2017. O-GlcNAc expression levels epigenetically regulate colon cancer tumorigenesis by affecting the cancer stem cell compartment via modulating expression of transcriptional factor MYBL1. J Biol Chem 292: 4123-4137. PMID:28096468

K Aoki-Kinoshita, S Agravat, NP Aoki, S Arpinar, RD Cummings, A Fujita, N Fujita, GM Hart, SM Haslam, T Kawasaki, M Matsubara, KW Moremen, S Okuda, M Pierce, R Ranzinger, T Shikanai, D Shinmachi, E Solovieva, Y Suzuki, S Tsuchiya, I Yamada, WS York, J Zaia, H Narimatsu. 2016. GlyTouCan 1.0--The international glycan structure repository. Nucleic Acids Res 44: D1237-D1242. PMID:26476458

R Berger, YH Sun, M Kulik, JK Lee, A Nairn, KW Moremen, M Pierce, S Dalton. 2016. ST8SlA4-dependent polysialylation is a part of a developmental program required for germ layer formation from human pluripotent stem cells. Stem Cells 34: 1742-1752. PMID:27074314

M Porterfield, P Zhao, H Han, J Cunningham, K Aoki, DD Von Hoff, MJ Demeure, JM Pierce, M Tiemeyer, L Wells. 2014. Discrimination between Adenocarcinoma and normal pancreatic ductal fluid by proteomic and glycomic analysis. J Proteome Res Feb 7; 13(2): 395-407. PMID:24328148

M. Porterfield, Z Peng, H Haiyong, J Cunningham, K. Aoki, D Von, J Demeure, M. Pierce, M. Tiemeyer, L. Wells. 2013. Discrimination between Adenocarcinoma and Normal Pancreatic Ductal Fluid by Proteomic and Glycomic Analysis. J. Proteome Res. In press.

P. Zhao, A.V. Nairn, S. Hester, K.W. Moremen, R.M. ORegan, G. Oprea, L. Wells, M. Pierce, K.L. Abbott. 2012. Proteomic identification of glycosylphosphatidylinositol anchor-dependent membrane proteins elevated in breat carcinoma. J.Biol Chem. 287: 25230-25240. PMID:22654114

A.V. Nairn, K. Aoki, M. Dela, M. Porterfield, J.M. Lim, M. Kulik, J.M. Pierce, L. Wells, S. Dalton, M. Tiemeyer, K.W. Moremen. 2012. Regulation of glycan structures in murine embryonic stem cells: Combined transcript profiling of glycan-related genes and glycan structural analysis. J. Biol. Chem. 287: 37835-37856. PMID:22988249

J.K. Lee, R.T. Matthews, J.M. Lim, K. Swanier, L. Wells, J.M. Pierce. 2012. Developmental expression of the neuron-specific N-caetylglucosaminyltransferase Vb (GnT-Vb/lX) and identification of its in vivo glycan products in comparison with those of its paralog, GnT-V . J.Biol. Chem. 287: 28526-28536. PMID:22715095

M.D. Langer, H. Guo, J.M. Pierce, D.E. Leckband. 2012. N-glycosylation alters cadherin-mediated intercellular binding. J. Cell. Sci. 125: 2478-2485. PMID:22344255

H.B. Guo, A. V. Nairn, M. Dela Rosa, T. Nagy, S. Zhao, K. Moremen, M. Pierce. 2012. Transcriptional regulation of the protocadherin beta cluster during Her-2 induced mammary tumorigenesis results from alter N-glycan branching. J. Biol. Chem. 287: 24941-24954. PMID:22665489

L.M. Tedaldi, M. Pierce, G.K. Wagner. 2012. Optimised chemical synthesis of 5-substituted UDP-sugars and their evaluation as glycosyltransferase inhibitors. Carbohydr.Res. 364: 22-27. PMID:23147042

K. Mo, T. Fang, S. Stainaker, P.S. Kirby, M. Liu, L. Wells, M. Pierce, D.H. Live, G.J. Boons. 2011. Synthetic, structural and biosynthetic studies of an unusual phosphoglycopeptide derived from α-dystroglycan. J. Am. Chem. Soc. 133: 14418-14430.

G. Alvarez-Manilla, N.L. Warren, J.A. Atwood, S. Dalton, R.C. Orlando, J.M. Pierce. 2010. Glycoproteomic analysis of embryonic stem cells: Identification of potential glycobiomarkers using lectin affinity chromatography of glycopeptides. J. Proteome Res. 9: 2062-2075. PMID:19545112

G. Alvarez-Manilla, K. Troupe, M. Fleming, E. Martinez-Uribe, M. Pierce. 2010. Comparison of the substrate specificities and catalytic properties of the sister N-acetylglucosaminyltransferases, GnT-V and GnT-Vb(IX).. Glycobiology 20: 166-174. PMID:19846580

K. Abbott, M. Pierce. 2010. Lectin-based glycoproteomic techniques for the enrichment and identification of potential biomarkers. Methods Enzymol. 480: 461-476. PMID:20816222

H.B. Guo, H. Johnson, M. Randolph, T. Nagy, R. Blalock, M. Pierce. 2010. Specific modification regulates early events in mammary carcinoma formation. Proc. Natl. Acad. Sci. (USA) 107: 21116-21121. PMID:21078982

K.L. Abbott, J. Lim, L. Wells, B.B. Benigno, J.F. McDonald, M. Pierce. 2010. Identification of candidate biomarkers with cancer-specific glycosylation in the tissue and serum of endometrioid ovarian cancer patients by glycoproteomic analysis. Proteomics 10: 470-481. PMID:19953551

R. Orlando, J.M. Lim, J.A. Atwood, P.M. Angel, M. Feng, G. Alvarez-Manilla, K.W. Moremen, W.S. York, M. Tiemeyer, J.M. Pierce, S. Dalton, R.L. Wells. 2009. IDAWG: Metabolic incorporation of stable isotope labels for quantitative glycomics of cultured cells. J. Proteome Res. 8: 3816-3823. PMID:19449840

K.L. Abbott, J.-M. Lim, R.L. Wells, B.B. Benigno, J.F. McDonald, J.M. Pierce. 2009. Identification of candidate biomarkers with cancer-specific glycosylation in the tissue and serum of endometrioid ovarian cancer patients by glycoproteomic analysis. Proteomics 10: 470-481. PMID:19953551

H.B. Guo, H. Johnson, M. Randolph, I. Lee, J.M. Pierce. 2009. Knockddown of GnT-V expression inhibits ligand-induced downregulation of the epidermal growth factor receptor and intracellular signaling by inhibiting receptor endocytosis. Glycobiology 19: 547-549. PMID:19225046

H.B. Guo, H. Johnson, M. Randolph, J.M. Pierce. 2009. Regulation of homotypic cell-cell adhesion by branched N-glycosylation of N-cadherin extracellular EC2 and EC3 domains. J. Biol. Chem. 284: 34986-34997. PMID:19846557

H.B. Guo, A. Vandersall-Nairn, K. Harris, M. Randolph, G. Alvarez-Manilla, K. Moremen, M. Pierce. 2008. Loss of expression of N-acetylglucosaminyltransferase Va results in altered gene expression of glycosyltransferases and galectins. FEBS Lett. 582: 527-535. PMID:18230362

K.L. Abbott, A.V. Nairn, E.M. Hall, M.B. Horton, J.F. McDonald, K.W. Moremen, D.M. Dinulescu, M. Pierce. 2008. Focused glycomic analysis of the N-linked glycan biosynthetic pathway in ovarian cancer. Proteomics 8: 3210-3220. PMID:18690643

A.V. Nairn, W.S. York, K. Harris, E.M. Hall, J.M. Pierce, K.W. Moremen. 2008. Regulation of glycan structures in animal tissues: transcript profiling of glycan-related genes. J. Biol. Chem. 283: 17298-17313. PMID:18411279

K.L. Abbott, K. Aoki, J.-M. Lim, M. Porterfield, R. Johnson, R.M. O'Regan, L. Wells, M. Tiemeyer, M. Pierce. 2008. Targeted glycoproteomic identification of biomarkers for human breast carcinoma. J. Proteome Res. 7: 1470-1480. PMID:18271524

K.L. Abbott, R.T. Matthews, M. Pierce. 2008. Receptor tyrosine phosphatase beta (RPTP beta) activity and signaling are attenuated by glycosylation and subsequent cell surface galectin-1 binding. J. Biol. Chem. 283: 33026-33035. PMID:18838383

G. Alvarez-Manilla, N.L. Warren, T. Abney, J.A. Atwood, P. Azadi, W.S. York, M. Pierce, R. Orlando. 2007. Tools for glycomics: relative quantitation of glycans by isotopic permethylation using 13CH3I. Glycobiology 17: 677-6787. PMID:17384119

M.S. Macnaughtan, M. Kamar, G. Alvarez-Manilla, A. Vernot, J. Glushka, J.M. Pierce, J.H. Prestegard. 2007. NMR structural chaacterization of substrates bound to N-acetylglucosaminyltranferase V. J. Mol. Biol. 366: 1266-1281. PMID:17204285

R Nash, L. Neves, R. Faast, M. Pierce, S. Dalton. 2007. The lectin DBA recognizes glycan epitopes on the surface of murine embryonic stem cells: a new tool for characterizing pluripotent cells and early differentiation. Stem Cells 25: 974-982. PMID:17170066

H.B. Guo, M. Randolph, M. Pierce. 2007. Inhibition of a specific N-glycosylation activity results in breast carcinoma cell invasiveness-related phenotypes: inhibition of epidermal growth factor-induced dephosphorylation of focal adhesion kinase (FAK). J. Biol. Chem. 282: 22150-22162. PMID:17537730

A.V. Nairn, A. Kinoshita-Toyoda, H. Toyoda, J. Xie, K. Harris, S. Dalton, M. Kulik, J.M. Pierce, T. Toida, K.W. Moremen, R.J. Linhardt. 2007. Glycomics of proteoglycan biosynthesis in murine embryonic stem cell differentiation. J. Proteome Res. 6: 4374-4387. PMID:17915907

G. Alvarez-Manilla, J.A. Atwood, Y. Guo, N.L. Warren, R. Orlando, M. Pierce. 2006. Tools for glycoproteomic analysis: size exclusion chromatography facilitates identification of tryptic glycopeptides with N-linked glycosylation sites. J. Proteome Res. 5: 701-708. PMID:16512686

S. Datta, M. Pierce, M.W. Datta. 2006. Perlecan signaling: Helping hedgehog stimulate prostate cancer growth. Int. J. Biochem. Cell Biol. 38: 1855-1861. PMID:16750652

K. Abbott, K. Troupe, I. Lee, M. Pierce. 2006. Integrin-dependent neuroblastoma cell adhesion and migration on laminin is regulated by expression levels of two enzymes in the O-mannosyl-linked glycosylation pathway, PomGnT1 and GnT-Vb. Exp. Cell Res. Epub. 312: 2837-2850. PMID:16857188

I. Lee, H.B. Guo, M. Kamar, K. Abbott, K. Troupe, J.K. Lee, G. Alvarez-Manilla, M. Pierce. 2006. N-acetylglucosaminyltransferase VB expression enhances beta-1 integrin-dependent PC12 neurite outgrowth on laminin and collagen. J. Neurochem. 97: 947-956. PMID:16606368

A. Venable, M. Mitalipove, I. Lyons, K. Jones, S. Shin, M. Pierce, S. Stice. 2005. Lectin binding profiles of SSEA-4 enriched, pluripotent human embryonic stem cell surfaces. BMC Develop. Biol. 5: 15-25. PMID:16033656

H.-B. Guo, I. Lee, B. Bryan, M. Pierce. 2005. Deletion of mouse embryo fibroblast N-acetylglucosaminyltransferase V stimulates a1b5 integrin expression mediated by the protein kinase C signaling pathway. J. Biol. Chem. 280: 8332-8342. PMID:15615721

L.S. Kelly, M. Kozak, T. Walker, M. Pierce, D. Puett. 2005. Lectin immunoassays using antibody fragments to detect glycoforms of human chorionic gonadotropin secreted by choriocarcinoma cells. Anal. Biochem. 338: 253-262. PMID:15745745

M. Kamar, G. Alvarez-Manilla, T. Abney, P. Azadi, V.S.K. Kolli, R. Orlando, K.V.S. Kolli, M. Pierce. 2004. Analysis of the site-specific N-glycosylation of b1,6N acetyl-glucosaminyltransferase V. Glycobiology 14: 583-592. PMID:15084511

J.K. Lee, L.G. Baum, K. Moremen, M. Pierce. 2004. The X-lectins: A new family with homology to the Xenopus laevis oocyte lectin XL-35. Glycoconjugate J. 21: 443-450. PMID:15750785

A. Pemberton, P. Knight, J. Gamble, W. Colledge, J.-K. Lee, M. Pierce, H. Miller, H.R.P. Miller. 2004. Innate BALB/c enteric epithelial responses to Trichinella spiralis: Inducible expression of a novel goblet cell lectin, Intelectin-2, and its natural deletion in C57BL/10 mice. J. Immunol. 173: 1894-1901. PMID:15265922

I. Lee, M. Skinner, H.B. Guo, A. Sujan, M. Pierce. 2004. Expression of the vacuolar H +-ATPase 16 KDa subunit results in the Triton X-100-insoluble aggregation of b1 integrin aggregation and reduction of its cell surface expression. J. Biol. Chem. 17: 53007-53014. PMID:15466867

H.-B. Guo, I. Lee, M. Kamar, M. Pierce. 2003. N-linked b(1,6) glycan branching levels regulate cadherin-associated cell-cell adhesion and intracellular signaling pathways. J. Biol. Chem. 278: 52412-52424. PMID:14561752

M. Kaneko, M. Kamar, W.-J. Zhang, J.-K. Lee, K. Moremen, M. Pierce. 2003. A novel N-acetylglucosaminyltransferase, GnT-VB, that synthesizes N-linked b(1,6) branched glycans. FEBS Lett. 554: 515-519. PMID:14623122

E. Arranz-Plaza, A.S. Tracy, A. Siriwardena, J.M. Pierce, G.J. Boons. 2002. High avidity, low affinity multivalent interactions and the block of polspermy in Xenopus laevis. J. Am. Chem. Soc. 124: 13035-13046. PMID:12405830

N. Fregien, M.J. Pierce. 2002. A point mutation in the Mgat5 ORF in BW5147 PHA2.1 cells causes truncation and inactivation. Glycobiology 12: 389-394.

H.B. Guo, I. Lee, M. Kamar, S. Akiyama, M. Pierce. 2002. Abberant N-glycosylation of b1 integrin causes reduced a5b1 integrin clustering and stimulates cell migration. Cancer Res. 62: 6837-6845. PMID:12460896

K. Alverez, C. Haswell, M. St.Clair, G.-S. Perng, M. Shorebah, M. Pierce, N. Fregien. 2002. Sequences of the mouse N-acetylglucosaminyltransferase V (Mgat5) mRNA and an MRNA expressed by an Mgat5-deficient cell line. Glycobiology 12: 389-394.

J.-K. Lee, M. Pang, J. Schnee, L. Baum, K. Moremen, M. Pierce. 2001. Human homologs of the Xenopus oocyte cortical granule lectin XL35. Glycobiology 11: 65-73. PMID:11181563

B. Zhang, M.M. Palcic, D.C. Schriemer, G. Alvarez-Manilla, M. Pierce, O. Hindsgaul. 2001. Frontal affinity chromatography coupled to mass spectrometry (FAC/MS) for screening mixtures of enzyme inhibitors. Anal. Biochem. 299: 173-182.

W.J. Zhang, L. Revers, M. Pierce, H. Schachter. 2000. Regulation of human b-1,2,N-acetylglucosaminyltransferase I gene (MGAT2) expression by transcription factors Ets-1 and-2. Biochem. J. 347: 511-518.

S. Di, J. Glushka, M. Pierce. 1999. Enzymatic synthesis of natural and ¹³C enriched linear poly-N-acetyllactosamines as ligands for galectin-1. Glycobiology 9: 353-364. PMID:10089209

S Di Virgilio, J Glushka, KW Moremen, M Pierce. 1999. Enzymatic synthesis of natural and 13C enriched linear poly-N-Acetyllactosamines as ligands for galectin-1. Glycobiology 9: 353-364. PMID:10089209

L. Chen, W.J. Zhang, N. Fregien, M. Pierce. 1998. The Her-2/neu oncogene stimulates the transcription of N-acetylglucosaminyltransferase V and expression of its cell surface oligosaccharide products. Oncogene 17: 1087-1093. PMID:9798679

P. Buckhaults, L. Chen, N. Fregien, M. Pierce. 1997. Transcriptional regulation of N-acetylglucosaminyltransferase V by the src oncogene. J. Biol. Chem. 272: 19575-19581. PMID:9235963

J.-K. Lee, P. Buckhaults, C. Wilkes, M. Teilhet, K. Moremen, M. King, M. Pierce. 1997. Cloning and expression of the Xenopus laevis oocyte lectin and characterization of its mRNA levels during early development. Glycobiology 7: 367-372. PMID:9147045

M. Pierce, P. Buckhaults, L. Chen, N. Fregien. 1997. Regulation of N-acetylglucosaminyltransferase V and Asn-linked oligosaccharide b(1,6) branching by a growth factor signaling pathway and effects on cell adhesion and metastatic potential. Glycoconj. J. 14: 623-630. PMID:9298695

N. Zhang, J. Perng, L. Chen, D. Puett, M. Pierce. 1997. Circular dichroism of N-acetylglucosaminyltransferase V and its substrate interactions. J. Biol. Chem. 272: 4225-4229. PMID:9020137

L. Chen, N. Zhang, B. Adler, J. Browne, N. Fregien, M. Pierce. 1995. Preparation of antisera to recombinant, soluble N-acetylglucosaminyltransferase V and its visualization in situ. Glycoconj. J. 12: 813-823. PMID:8748159

M. Fregien, G.-S. Perng, M. Shoreibah, M. Pierce. 1995. Expression of N-acetylglucosaminyltransferase V mRNA in mammalian tissues and cell lines. Glycobiology 4: 867-872.

M. Granovsky, C. Fode, C.E. Warren, R.M. Campbell, J.D. Marth, M. Pierce, N. Fregien, J.W. Dennis. 1995. GlcNAc-Transferase V and core 2 GlcNAc-O-transferase expression in the developing mouse embryo. Glycobiology 5: 797-806. PMID:8720078

J.-K. Lee, M. Pierce. 1995. Purification and characterization of human serum N-acetylglucosamine-1-phosphodiester a-N-acetylglucosaminidase. Arch. Biochem. Biophys. 319: 413-425. PMID:7786023

P.A. Prieto, P. Mukerjit, B. Kelder, R. Erney, D. Gonzalez, J.S. Yun, D.F. Smith, K.W. Moremen, C. Nardelli, M. Pierce, Y. Li, X. Chen, T.E. Wagner, R.D. Cummings, J.J. Kopchick. 1995. Remodeling of mouse milk glycoconjugates by transgenic expression of a human glycosyltransferase. J. Biol. Chem. 270: 29515-29519.

C. Wilkes, M. Pierce. 1995. Glycosyltransferase assay system utilizing acylated biantennary glycopeptides. Anal. Biochem. 224: 174-179. PMID:7710067

K.Y. Do, N. Fregien, M. Pierce, R. Cummings. 1994. Activity of N-acetylglucosaminyl-transferase V toward glycoprotein acceptors: glycosylation by GlcNAc-T V is determined by its accessibility of oligosaccharides. J. Biol. Chem. 269: 23456-23464.

M.M. Palcic, M. Pierce, O. Hindsgaul. 1994. Neoglycoconjugates used in enzymatic assays and affinity supports. Methods Enzymol. 247: 215-227.

M. Shoreibah, G.-S. Perng, B. Adler, J. Weinstein, R. Basu, R. Cupples, D. Wen, J. Browne, P. Buckhaults, N. Fregien, M. Pierce. 1993. Isolation and expression of a rat cDNA encoding N-acetylglucosaminyltransferase V. J. Biol. Chem. 268: 15381-15385.

M. Shoreibah, M. Pierce. 1992. Purification and characterization of N-acetylglucosaminyltransferase-V from rat kidney. J. Biol. Chem. 267: 2920-2927.

J. Drazba, M. Pierce, V. Lemmon. 1991. Studies of the developing chick retina using monoclonal antibody 8A2 that recognizes a novel set of gangliosides. Dev. Biol. 145: 154-163.

S.C. Crawley, O. Hindsgaul, G. Alton, M. Pierce, M.M. Palcic. 1990. An enzyme-linked immunosorbent assay for N-acetylglucosaminyltransferase-V. Anal. Biochem. 185: 112-117.

M.M. Palcic, J. Ripka, K.J. Kaur, M. Shoreibah, O. Hindsgaul, M. Pierce. 1990. Regulation of N-acetylglucosaminyltransferase V activity; kinetic comparisons of parental, Rous sarcoma virus-transformed BHK, and L-phytohemagglutinin-resistant BHK cells using synthetic substrates and an inhibitory substrate analog. J. Biol. Chem. 265: 6759-6769.

J. Ripka, M. Pierce, N. Fregien. 1990. Co-transformation of Lecl CHO cells with N-acetylglucosaminyltransferase I activity and a selectable marker. J. Cell. Biochem. 42: 1-6.

J. Ripka, M. Pierce, N. Fregien. 1989. DNA-mediated transformation of N-acetylglucosaminyltransferase I activity into an enzyme deficient cell line. Biochem. Biophys. Res. Commun. 159: 554-560.

J. Arango, M. Pierce. 1988. Comparison of N-acetylglucosaminyltransferase V activities in Rous sarcoma-transformed baby hamster kidney (RS-BHK) and BHK cells. J. Cell. Biochem. 37: 225-231.

O. Hindsgaul, S.H. Tahir, O.P. Srivastava, M. Pierce. 1988. The trisaccharide b-D-GlcNAc(1,2)b-D-man(1,6)a-D-man, as its 8-methyoxycarbonyloctyl glycoside, is an acceptor selective for N-acetylglucosaminyltransferase V. Carbohydr. Res. 173: 263-272.

M.M. Palcic, L.D. Heerze, M. Pierce, O. Hindsgaul. 1988. The use of hydrophobic synthetic glycosides as acceptors in glycosyltransferase assays. Glycoconj. J. 5: 49-63.

O.P. Srivastava, O. Hindsgaul, M. Shoreibah, M. Pierce. 1988. Recognition of oligosaccharide substrates by N-acetylglucosaminyltransferase V. Carbohydr. Res. 179: 137-161.

M. Pierce, J. Arango, S.H. Tahir, O. Hindsgaul. 1987. Activity of UDP-GlcNAc:a-mannoside b(1,6)N-acetylglucosaminyltransferase (GnT V) in cultured cells. Biochem. Biophys. Res. Commun. 146: 679-684.

L. Bourguignon, K. Balazovich, S. Suchard, O. Hindsgaul, M. Pierce. 1986. Endocytosis of mannose-6-phosphate binding sites by T-lymphoma cells. J. Cellular Physiol. 127: 146-161.

M. Pierce, J. Arango. 1986. Rous sarcoma virus-transformed baby hamster kidney cells express higher levels of asparagine-linked tri- and tetraantennary glycopeptides containing [GlcNAc(b)(1,6)Man] and poly N-acetyllactosamine sequences than baby hamster kidney cells. J. Biol. Chem. 261: 10772-10777.

P. Szylagi, J. Arango, M. Pierce. 1985. Separation of metabolically radiolabeled O-methylmannitols and O-methylfucitol by reverse-phase high performance liquid chromatography: Applications to animal cell oligosaccharide structural analysis. Anal. Biochem. 148: 260-267.

Book Chapters

J.M. Pierce. 2009. Cancer glycomics. In: Handbook of Glycomics (R.D. Cummings and J.M. Pierce, eds.), pp. 397-429. Academic Press.

To Top