UGA-GRA 800 MHz NMR Regional Facility
|
|
The Georgia Research Alliance and the University of Georgia have established a
regional NMR facility located at the Complex
Carbohydrate Research Center that is directed by Professor
James Prestegard. Its purpose is to provide a high-field NMR spectroscopy
laboratory that is accessible primarily to the Georgia research community and
other investigators in the region.
The 800 MHz NMR spectrometer is especially applicable to cases in which
normally weak field-dependent phenomena become observable. It can also provide
the additional resolution and sensitivity needed in particularly demanding
problems of structure determination, and is another frequency available for
relaxation experiments and other studies. We currently have a triple
resonance cold probe with a flow-cell option, as well as
standard liquid probes and a variable angle solids probe.
Lower field magnets (500WB,600) are also available on-site in the CCRC NMR
Spectroscopy Center, for preliminary work, sample testing, and pulse
program optimization.
Spectrometer description
|
Magnet |
63mm bore |
2.2 Kelvin |
|
|
|
Console |
Varian Inova |
4 RF channels 3 waveform boards |
3 axis gradients z-grad waveform board |
|
|
Probes |
NEW! 5mm H{CN} cold probe ; flow-cell option |
5mm H{CN} rt |
5mm C{HN} rt |
Doty VAS triple resonance solids probe |
|
Workstations, etc. |
Sunblade 1500 |
Storage to CD, DVD or tape |
Offline workstations for data processing (NMRPipe, Felix) |
Remote access |
For technical questions please contact John Glushka; 706.542.4483 glushka@ccrc.uga.edu
Guidelines for
access to the GRA-UGA 800 MHz NMR
Priority to high-field applications.
This facility currently emphasizes the determination of structures of biologically
important macromolecular complexes, and the use of field-induced orientation
phenomena. Priority will be given to the applications that take advantage of
the special capabilities of the facility. Priority will also be given to
researchers located in the state of Georgia.
A user-run facility.
Our goal is to run a facility in which experienced users assume responsibility
for most of their own data acquisition. However, assistance may be had from the
CCRC analytical services staff (please contact the facility manager, John Glushka)
or by collaboration with trained users. A list of users from Georgia NMR
Research Groups and their areas of expertise is provided below.
Adequate training of independent users is required and can be obtained either
at research universities in Georgia or at the CCRC, using lower field
instruments with Varian consoles. NMR facility managers at these universities
can certify the level of experience of potential users. For those not familiar
with Varian software and for specific details pertinent to the facility,
on-site training will be provided without charge. Final evaluation of
spectrometer users will be made by the manager.
Remote operation.
It is possible to operate the spectrometer remotely and retrieve data. Subject
to pre-arrangement with CCRC personnel, samples may be sent in NMR tubes with
appropriate hazardous material documentation to the facility. Samples will be placed
in the spectrometer and the hardware configured appropriately.
Submitting projects
and Scheduling NMR time
Initiating a project
A researcher should submit a short written request to the facility manager.
The request should describe the nature of the project and the need for high
field, an estimate of the required time, a proposed funding source, and whether
the user will require assistance. The request should be accompanied by an
example of data acquired at lower field on the system to be studied (e.g. a 1H-15N
HSQC spectrum ), and when applicable, a certification of user competence from a
local facility manager. For non-standard pulse programs, a version, along with
a suitable parameter set, should be provided well in advance for optimization
on the 800 MHz spectrometer.
The request is reviewed by the director, Prof. J. Prestegard. Approvals and
rejections are reviewed semi-annually by the facility advisory committee.
Appeals of any rejections may be addressed to either Dr. Prestegard or the
co-chair of the facility advisory committee, Dr. Dabney Dixon.
Publications using data from the facility should contain an acknowledgment of
facilities provided by the GRA Biotechnology Center, and the facility staff
should be provided with a list of those publications. Approvals normally expire
after one year, but may be renewed upon written request.
Scheduling procedure
After approval, initial training and/or experiment time should be scheduled
with the manager. Approved users can subsequently schedule time through a
Web-based NMR scheduling calendar. The
user is expected to use time responsibly and will be billed for time scheduled.
The manager may need to adjust time allocations based on demand, project
priorities, and other circumstances, and users will be notified in a timely
fashion whenever possible.
Charges for usage
Users are responsible for support of the facility though the payment of fees
for spectrometer time. Charges will be based on time reserved, regardless of
the success of the experiment, barring technical problems of the facility.
Payment for operator assistance, or authorship for collaborations should also
be arranged. Academic and
non-profit rates are listed below. Interested corporate users should contact Dr. Parastoo Azadi to initiate a service
contract.
|
|
Spectrometer
time |
Operator time |
|
Academic (in
state) |
$13.00 |
$40.00 |
|
Academic (out of
state) |
$18.00 |
$50.00 |
|
Corporate |
Please contact Dr.P.Azadi |
Please contact Dr. P.Azadi |
Special arrangements can be made for extended periods or development work.
Spectrometer training is free to users.
Facility Advisory
Committee
Co-Chairs:
James
Prestegard, Varian-GRA Eminent Scholar in NMR, Professor,
Departments of Chemistry, and Biochemistry and Molecular Biology, and Complex
Carbohydrate Research Center, UGA
220 Riverbend Road., Athens, GA 30602-4712
Tel: 706.542.6281 jpresteg@ccrc.uga.edu
Dabney Dixon, Associate Professor, Dept. of Chemistry, Georgia State
University,
Tel: 404.651.3908 chedw@panther.gsu.edu
Other members:
Robert A. Scott,professor, Department of Chemistry, University of Georgia
Professor Suzzane B. Shuker, School of Chemistry and Biochemistry, Georgia
Institute of Technology
Professor Yitibarek Habte-Mariam, Dept. of Chemistry Clark Atlanta University
Professor William Dynan, Dept. of Molecular Medicine, Medical College of
Georgia
Users
For those who need assistance in acquiring data on the 800MHz spectrometer,
please contact the following trained users from Georgia research groups for
collaborative arrangements.
|
Name |
Location |
Contact |
Comments |
|
John Glushka,facility manager |
CCRC,UGA |
glushka@ccrc.uga.edu 706.542.4483 |
carbohydate,nD protein, general organic |
|
Quincy Teng,facility manager |
Chemistry, UGA |
teng@sunchem.chem.uga.edu 706.542.1502 |
nD protein, general organic |
|
Hsiau-We Lee (Jacques),graduate student |
GA State |
2D protein |