CCRC NMR Spectroscopy Facility
|
|
CCRC NMR Facility
The CCRC NMR spectroscopy facility, under the direction of Professor James H. Prestegard, is used to
determine molecular structures of carbohydrates and proteins and to investigate
the structural and dynamical basis of protein-carbohydrate interactions. The
CCRC NMR Facility consists of three high-field Varian spectrometers (a Mercury 300 MHz,
an Inova 500 MHz wide-bore, and an Inova 600 MHz) equipped for high-resolution liquids and semi-solids experiments. To
initiate an analytical service contract, please contact Parastoo Azadi, 706-542-0629. Technical
inquiries about the facility or NMR services should be directed to John Glushka, 706-542-4483.
GRA-UGA 800 MHz NMR Regional Facility
The GRA-UGA 800 MHz NMR Regional
Facility is located at the CCRC and is available to researchers seeking the
advantages of high-field systems ( field-induced orientation, high sensitivity
and spectral dispersion). NEW! Cold probe installed with flow cell
option.
NEW! Southeast Collaboratory for
High-field Biomolecular NMR 900
MHz spectrometer
The SECNMR 900 MHz spectrometer has been
recently installed at the CCRC. Please follow the link for further information.
Spectrometer descriptions
The 300 MHz can be used
routinely for both proton and carbon observe, but requires a probe change for
P31. The 500 requires a probe change for C13 or P31 observe. The 600 requires a
probe change for automated sample handling with either a conventional flow
probe or the CapNMR microflow probe.
|
Spectrometer |
300 MHz |
500 MHz(wide bore) |
600 MHz |
|
Console |
Varian Mercury |
Varian Inova |
Varian Inova |
|
RF Channels |
2 |
3 |
4 |
|
Gradients |
z |
z |
z |
|
Probes |
5mm X{H} ATP |
5mm H{CN} |
5mm H{CN} |
Laboratory facilities, workstations, and software
Standard laboratory facilities and supplies are available for sample preparation.
SUN and PC workstations running Felix (MSI), VNMR and NMRPipe are available for
data processing. Data storage to CD, DVD or 4mm DAT tape is available on site.
Facility policies
NMR data can be obtained via a service or a collaborative arrangement with
CCRC personnel, or directly by researchers after adequate training on the
spectrometers. Training will be provided free of charge on the 300MHz
instrument. We reserve the right to confer or rescind spectrometer privileges
dependent on training level, time elapsed since last session, and treatment of
the spectrometer. The qualified user will be able to reserve time using FACES, our web based scheduling program.
Charges for usage
Qualified users will be
charged for the time scheduled on the spectrometer. For service work or
assistance additional operator charges apply and a quote will be provided. For
collaborative projects intended for co-authored publications, specific
arrangements should be made with the appropriate CCRC faculty or staff member.
The following rates apply to
“routine” samples of sufficient purity and amount, and with adequate supporting
information (e.g. known literature data) for a straightforward analysis. Beyond
simple 1D proton data, a typical sample might require a few 2D experiments and
a day of analysis. More difficult samples will typically require an initial
evaluation and some discussion of the cost estimate. Sessions that require probe changes or additional hardware
setup will be charged accordingly.
Rates[US
$ /Hour]
|
|
Cost Category |
300 MHZ |
500 MHz |
600 MHZ |
Minimum |
|
Academic |
Instrument |
3 |
5 |
6 |
|
|
Corporate |
Instrument |
50 |
50 |
50 |
300 |
Analytical Services and Sample Requirements
Samples should be pure and contain < 50 mM aprotic salts. Mixtures and
impure samples may be assessed qualitatively, but generally are not suitable
for NMR analysis. For very high MW polysaccharides, broad lines in the spectra
may hinder the analysis. We recommend that a lower molecular weight fragment
(e.g. repeating unit, backbone without side chains, de-acetylated, etc.) also
be provided if possible. To reduce the time for data analysis, any supporting
literature data and/or analogous compounds should accompany the sample.
|
|
1D Proton |
2D Proton
(e.g.TOCSY) |
1D , 2D Carbon
(e.g.HSQC) |
|
Analysis |
Estimate purity or major components in mixtures. |
Identify residues and linkages. |
1D: Identify expected cmpd. |
|
Amounts |
~50 nanomoles or ~10 ug per sugar residue (.eg. 60 ug for hexasaccharide) |
~100 nanomoles |
1 micromole |
|
Approx. time* |
30 min |
4 hours per 2D |
8 hours |
*On 500MHz, does not include operator time.
Research Groups
The following research groups are primary users of the NMR facility. Initial
contact should be with the facility manager, however, collaborations or other
inquiries may be directed to specific researchers.
|
Contact |
Research Programs,
Areas of Interest |
Research Groups
|
|
James
H.Prestegard |
Development of
NMR methods for determining structures of proteins and carbohydrates, and
their interactions. |
|
|
Will York |
Structural
analysis of plant cell walls. |
|
|
Russell W.
Carlson |
Molecular basis
of the interaction between a bacterium and its plant or animal host.
Structures of Lipopolysaccharides. |
|
|
Robert J. Woods
|
Development and
Application of Computational Methods. |
|
|
Geert-Jan Boons
|
Synthesis of
Oligosaccharides. |
Southeast Collaboratory for High-field
biomolecular NMR (SECNMR) 900 MHz spectrometer
UGA-GRA 800 MHz NMR Regional
Facility.
Southeast Collaboratory for Structural
Genomics (SECSG); Dr. Kristen Mayer
Dept. of Chemistry; Dr. Quincy Teng
Dept. of
Biochemistry and Molecular Biology; Prof. J. Urbauer