Publications

M. Xie, G. Krooshof, J. Benen, J. Atwood, D. King, C. Bergmann, R. Orlando. 2005. Post-translational Modifications of Recombinant B. Cinerea EPG 6. Rapid. Commun. Mass. Spectrom. 19(22), 3389-97.

J. Atwood, S. Sahoo, G. Alvarez-Manilla, D. Weatherly, K. Kolli, R. Orlando, W. York. 2005. Simple modification of a protein database for mass spectral identification of N-linked glycopeptides. Rapid. Commun. Mass. Spectrom. 19(21), 3002-6.

J. Atwood, D. Weatherly, T. Minning, B. Bundy, C. Cavola, F. Opperdoes, R. Orlando, R. Tarleton. 2005. The Trypanosoma cruzi proteome. Science . 309(5733), 473-476.

D. Weatherly, J. Atwood, T. Minning, C. Cavola, R. Tarleton, R. Orlando. 2005. A heuristic method for assigning a false-discovery rate for protein identifications from Mascot database search results. Mol. Cell. Proteomics . 4(6), 762-772.

M. Kamar, G. Alvarez-Manilla, T. Abney, P. Azadi, V.S. Kolli, R. Orlando, M. Pierce. 2004. Analysis of the site-specific N-glycosylation of beta 1,6N acetyl-glucosaminyltransferase V. Glycobiology 14 : 583-592.

M.J. Sheehy, J.F. Gallagher, M. Yamashita, Y. Ida, J. White-Colangelo, J. Johnson, R. Orlando, P.T.M. Kenny. 2004. Synthesis and structural characterization of redox-responsive N-ferrocenoyl amino acid esters; the X-ray crystal structure of N-ferrocenoyl alanine methyl ester; electrochemical anion recognition and 1H NMR complexation studies. J. Organometallic Chem. 689 : 1511-1520.

L. Feng, R. Orlando, J. Prestegard. 2004. Mass spectrometry assisted assignment of NMR resonances in 15 N labeled proteins. J. Am. Chem. Soc. 126 : 14377-14379.

M.E. Warren, H.C.M. Kester, J. Benen, J. Colangelo, J. Visser, C. Bergmann, R. Orlando. 2002. Studies on the glycosylation of wild-type and mutant forms of Aspergillus niger pectin methylesterase. Carbohydr. Res. 227 : 803-812.

D. King, C. Bergmann, R. Orlando, J.A.E. Bensen, H.C.M. Kester, J. Visser. 2002. The use of amide exchange-mass spectrometry to study conformational changes within the endopolygalacturonase II/homogalacturonan/polygalacturonase-inhibiting protein system. Biochemistry 41 : 10225-10233.

D. King, M. Lumpkin, C. Bergmann, R. Orlando. 2002. Studying protein-carbohydrate interactions by amide hydrogen/deuterium exchange mass spectrometry. Rapid Commun. Mass Spectrom. 16 : 1569-1574.

L.P. Kotra, L. Zhang, R. Fridman, R. Orlando, S. Mobashery. 2002. N-Glycosylation pattern of the zymogenic form of human matrix metalloproteinase-9. Biorg. Chem 30 : 356-370.

J. Watras, R. Orlando, I.I. Mararu. 2000. An endogenous sulfated inhibitor of neuronal inositol trisphosphate receptors. Biochemistry 39 : 3452-3460.

H.C.M. Kester, J.A.E. Benen, J. Visser, M.E. Warren, R. Orlando, C. Bergmann. 2000. Tandem mass spectrometric analysis of Aspergillus niger pectin methylesterase: mode of action on fully methylesterified oligogalacturonates. Biochem. J. 346 : 469-474.

L.J. Gonzalez, T. Takao, T. Shimizu, Y. Satomi, L. Betancourt, V. Besada, G. Padron, R. Orlando, T. Shirasawa, Y. Shimonishi. 2000. Differentiating alpha- and beta-aspartic acids by low-energy electrospray ionization mass spectrometry. Rapid Commun. Mass Spectrom. 14: 2092-2102.

R. Orlando, A.H. Brockman, R.L. Tarleton. 2000. Identificacion de peptidos asociados al MHC de celulas infectadas por Trypanosoma cruzi mediante cromatografia liquida/espectrometria de masas en tandem. Biotecnologia Aplicada 17: 193-199.

A.H. Brockman, R. Orlando, R.L. Tarleton. 1999. A new liquid chromatography/tandem mass spectrometric approach for the identification of class I major histocompatibility complex-associated peptides that eliminates the need for bioassays. Rapid Commun. Mass Spectrom. 13: 1024-1030.

J. Colangelo, V. Licon, J. Benen, C. Bergmann, R. Orlando. 1999. Characterization of the glycosylation of recombinant endopolygalacturonase I from Aspergillus niger. Rapid Commun. Mass Spectrom. 13: 1448-1453.

J. Colangelo, R. Orlando. 1999. On-target exoglycosidase digestions/MALDI-MS for determining the primary structures of carbohydrate chains. Anal. Chem. 71: 1479-1482.

J. Colangelo, V. Licon, J. Benen, J. Visser, C. Bergmann, R. Orlando. 1999. Characterization of the N-linked glycosylation site of recombinant pectate lyase. Rapid Commun. Mass Spectrom. 13: 2382-2387.

L. Zhang, R. Orlando. 1999. Solid-phase extraction/MALDI-MS: extended ion-pairing surfaces for the on-target clean-up of protein samples. Anal. Chem. 71: 4753-4757.

A.H. Brockman, N.N. Shah, R. Orlando. 1998. Optimization of a hydrophobic solid-phase extraction interface for matrix-assisted laser desorption/ionization. J. Mass Spectrom. 33: 1141-1147.

V.S.K. Kolli, W.S. York, R. Orlando. 1998. Fast atom bombardment-mass spectrometry of carbohydrates contaminated with inorganic salts using crown ethers. J. Mass Spectrom. 33: 680-682.

M.E. Warren, A.H. Brockman, R. Orlando. 1998. On-probe solid-phase extraction/MALDI-MS using ion-pairing interactions for the clean-up of peptides and proteins. Anal. Chem. 70: 3757-3761.

Y. Yang, K.D. Arbtan, K.P. Carmichael, R. Orlando. 1998. Is canine hepatocerebellar degeneration syndrome an animal model for carbohydrate-deficient glycoprotein syndrome in humans? An example of sequencing glycoprotein glycans with mass spectrometry. Rapid Commun. Mass Spectrom. 12: 571-579.

Y. Yang, J. Roestamadji, S. Mobashery, R. Orlando. 1998. The use of neamine as a molecular template: identification of the active site residues in the bacterial antibiotic resistance enzyme aminoglycoside 3?-phosphotransferase type IIa by mass spectrometry. Bioorg. Med. Chem. Lett. 8: 3489-3494.

A.H. Brockman, B.S. Dodd, R. Orlando. 1997. A desalting approach for MALDI-MS using on-probe hydrophobic self-assembled monolayers. Anal. Chem. 69: 4716-4720.

E. Chpak, V.S.K. Kolli, R. Orlando, M. Kieliszewski. 1997. Glycosylation specifics of the archetypal SER-HYP-HYP-HYP-HYP block of tomato extensin P1. Plant Physiol. 114: 331-.

S.C. Jao, K. Ma, T. Talafous, R. Orlando, M.G. Zagorski. 1997. Trifluoroacetic acid pretreatment reproducibly disaggregates the amyloid b-peptide. Amyloid: Int. J. Exp. Clin. Invest. 4: 240-252.

P.T.M. Kenny, K. Nomoto, R. Orlando. 1997. Fragmentation studies of gas-phase complexes between alkali metal ions and a biologically active peptide, achatin-I. Rapid Commun. Mass Spectrom. 11: 224-227.

M. Kieliszewski, R. Orlando. 1997. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry of tomato extensin monomers and potato lectin. Phytochemistry 45: 9-14.

V.S.K. Kolli, R. Orlando. 1997. A new strategy for MALDI on magnetic sector mass spectrometers with point detectors. Anal. Chem. 69: 327-332.

Y. Yang, C. Bergmann, J. Benen, R. Orlando. 1997. Site-specific structural characterization of the glycans of recombinant endopolygalacturonase II (EPG-II) by mass spectrometry. Rapid Commun. Mass Spectrom. 11: 1257-1262.

A. Brockman, R. Orlando. 1996. Calculating empirical formulas. Anal. Chem. 68: 484A-485A.

A. Brockman, R. Orlando. 1996. New immobilization chemistry for probe affinity mass spectrometry. Rapid Commun. Mass Spectrom. 10: 1688-1692.

V.S.K. Kolli, R. Orlando. 1996. A new matrix for MALDI on magnetic sector instruments with point detectors. Rapid Commun. Mass Spectrom. 10: 923-926.

R. Orlando, S. He, V.S.K. Kolli, K. Wu, S. Pan. 1996. The primary structure of human ?B1-crystallin from normal lenses. Invest. Opthalmol. Vis. Sci. 37: S597-.

T.J.W. Stokkermans, R. Orlando, V.S. Kumar Kolli, R.W. Carlson, N.K. Peters. 1996. Biological activities and structures of Bradyrhizobium elkanii low abundance lipo chitin-oligosaccharides. Mol. Plant-Microbe Interac. 9: 298-304.

Y. Yang, R. Orlando. 1996. Simplifying the exoglycosidase digestion/MALDI-MS procedures for sequencing N-linked carbohydrate side chains. Anal. Chem. 68: 570-572.

Y. Yang, R. Orlando. 1996. Identifying the glycosylation sites and site-specific carbohydrate heterogeneity of glycoproteins by matrix-assisted laser desorption/ionization mass spectrometry. Rapid Commun. Mass Spectrom. 10: 932-936.

W.S. York, V.S.K. Kolli, R. Orlando, P. Albersheim, A.G. Darvill. 1996. The structures of arabinoxyloglucans produced by solanaceous plants. Carbohydr. Res. 285: 99-128.

A. Brockman, R. Orlando. 1995. Probe-immobilized affinity chromatography/mass spectrometry. Anal. Chem. 67: 4581-4585.

B. Francis, J. Schmidt, Y. Yang, R. Orlando, I.I. Kaiser. 1995. Anions and the curious gel filtration behavior of notexin and scutoxin. Toxicon. 33: 779-789.

S. He, S. Pan, K. Wu, I.J. Amster, R. Orlando. 1995. Analysis of normal human fetal eye lens crystallins by high performance liquid chromatography/mass spectrometry. J. Mass Spectrom. 30: 424-431.

M.J. Kieliszewski, M.A. O'Neill, J. Leykam, R. Orlando. 1995. Tandem mass spectrometry and structural elucidation of glycopeptides from a hydroxyproline-rich plant cell wall glycoprotein indicate that contiguous hydroxyproline residues are the major sites of hydroxyproline O-arabinosylation. J. Biol. Chem. 270: 2541-2549.

K.V.S. Kolli, R. Orlando. 1995. Complete sequence confirmation of large polypeptides by high energy collisional activation of multiply protonated ions. J. Am. Soc. Mass Spectrom. 6: 234-241.

R. Orlando, S. He, K. Wu, S. Pan. 1995. Comparison of the water-soluble crystallins from normal and age-related cataractous human lenses. Invest. Opthalmol. Vis. Sci. 36: S626-.

R. Orlando, Y. Yang. 1995. Glycosylation site mapping by MALDI mass spectrometry. Glycoconj. J. 12: 402-.

D.A. Powell, R. Orlando, J. Travis, H. Van Halbeek. 1995. N-glycosylation site mapping of human plasma ?1-antichymotrypsin. Glycoconj. J. 12: 411-.

A.J. Alpert, M. Shukla, A.K. Shukla, L.R. Zieske, S.W. Yuen, M.A.J. Ferguson, A. Mehlert, M. Pauly, R. Orlando. 1994. Hydrophilic-interaction chromatography of complex carbohydrates. J. Chromatogr. A. 676: 191-202.

R. Ubillas, S.D. Jolad, R.C. Bruening, M.R. Kernan, S.R. King, D.F. Sesin, M. Barrett, C. Stodart, T. Flaster, J. Kuo, F. Ayala, E. Mesa, E. Rozhon, M.S. Tempesta, D. Barnard, J. Huffman, D. Smee, R. Sidwell, S. Spruance, M. McKeough, A. Brazier, S. Safrin, R. Orlando, P.T.M. Kenny, N. Berova, K. Nakanishi. 1994. SP303, an antiviral agent from Sangre de grado. Phytomedicine 1: 77-106.

K.A. Lerro, R. Orlando, H. Zhang, P.N.R. Usherwood, K. Nakanishi. 1993. High-performance liquid chromatographic systems for the separation of "sticky" peptides from membranes. Anal. Biochem. 215: 38-44.

R. Orlando, P.T.M Kenny, C. Moquin-Pattey, K.A. Lerro, K. Nakanishi. 1993. Sequencing membrane proteins by tandem mass spectrometry. Org. Mass Spectrom. 28: 1395-1402.

Book Chapters

S.C. Wu, J. Johnson, A.G. Darvill, P. Albershiem, R. Orlando. 2004. Proteomics of Magnaporthe grisea: liquid chromatography mass spectrometry for the identification of extracellular proteins . In: Rice Blast Disease: Interaction with Rice and Control (S. Kawasaki, ed.), pp. 39-46. Kluwer Press, Dordrecht, The Netherlands.

C.W. Bergmann, L. Stanton, D. King, R.P. Clay, G. Kemp, R. Orlando, A. Darvill, P. Albersheim. 2003. Recent observations on the specificity and structural conformation of the polygalacturonase-polygalacturonase inhibiting protein system. In: Advances in Pectin and Pectinase Research (F. Voragen, H. Schols, and R. Visser, eds.), pp. 277-291. Kluwer Academic Publishers, The Netherlands.

V.S.K. Kolli, J. Johnson, R. Orlando, A.G. Darvill, P. Albersheim, S.-C. Wu. 2003. Proteomic identification of extracellular proteins secreted by the rice blast fungus. In: Proceedings of the 51st American Society of Mass Spectrometry Conference, Montreal, Canada, June 8-12.

R. Orlando, Y. Yang. 1998. Analysis of glycoproteins. In: Mass Spectrometry of Biological Materials (B.S. Larsen and C.N. McEwen, eds.), pp. 215-246. New York: Marcel Dekker, Inc..

R. Orlando, Y. Yang. 1997. Picoscale sequencing of glycoprotein glycans with mass spectrometry. In: Proceedings of the Seventh National Symposium on Mass Spectrometry (S.K. Aggarwal and H.C. Jain, eds.), pp. 145-157. Indian Society for Mass Spectrometry.