Welcome to the Dr. Michael Pierce Lab!

Current Lab Members

Jin Kyu Lee
Position: Research Assistant
Office: (706) 542-0514
Lab: (706) 542-1701
Email: jinkyu@uga.edu
Current project: Characterization of HL1/2
and GnT-VB regulation

Education and Honors:
1979-1984 B.S Yonsei University, Biochemistry, Seoul, Korea
1985-1986 M.S Yonsei University, Biochemistry, Seoul, Korea
1992-1997 Ph.D University of Georgia, Biochemistry and Molecular Biology
1997-2000 Post-doc, University of California, San Francisco, Immunology program
1997-2000 National Arthritis Foundation Postdoctoral Fellowship, UCSF
2001-present Research Assistant Professor, Dept. of Biochemistry, University of Georgia
Selected Publications:

Jin Kyu Lee, Linda G. Baum, Kelly Moremen and Michael Pierce. The
X-Lectins: a new family with homology to the Xenopus laevis oocyte
lectin XL-35. Glycoconjugate Journal, Review; Glycoconjugate Journal
21:443-450 review
Jin Kyu Lee, Annette Bistrup, Annemieke van Zante, and Steven D. Rosen.
Activities and expression pattern of the carbohydrate sulfotransferase, GlcNAc6ST-3
(I-GlcNAc6ST): Functional Implications. Glycobiology, 2002;13, 245-254

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Gerardo Alvarez-Manilla
Position: Research Assistant
Lab: (706) 542-1701
Email: alvarez@bmb.uga.edu
Current project: Glycomics of stem cells
and tumor cell surface carbohydrate

Education and Honors:
1984-1987 B.S, ITESM-Campus Guaymas, Biochemical Engineering, Mexico
1989-1991 M.S, ITESM-Campus Guaymas, Marine Biology
1991-1995 Ph.D, University of Georgia, Biochemistry
1995-1997 Postdoc, CCRC, University of Georgia
1997-2000 Research Scientist and coordinator, Research Center for Food and Development, Mexico
2001-present Co-Technical Director, NIH Integrated Resorce Center for Glycomics, CCRC, University of Georgia
Selected Publications:

Valadez-Vega, M. C., Guzman-Partida, A. M., Soto-Cordova, F. J., Azadi,
P. and Alvarez-Manilla, G. Purification, biochemical characterization
and carbohydrate specificity of a lectin purified from the seeds of the
tepary bean lectin Phaseolus acutifolius A.Gray. Journal of the
Sciences of Food and Agriculture. Manuscript submitted.

Maria Kamar, Gerardo Alvarez-Manilla, Trina Abney, Parastoo Azadi,
V.S. Kumar Kolli, Ron Orlando, and Michael Pierce. Analysis of the site
specific N-glycosylation of b(1,6)N-acetylglucosaminyltransferase V.
Glycobiology. In press.

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Huabei Guo
Position: Research Assistant
Lab: (706) 542-1701
Email: hbguo@uga.edu
Current project: Effect of tumor cell specific
glycosylation (GnT-VA,B) on cell adhesion
receptors, integrin and cadherin

Education and Honors:
1980-1985 B.S Bengbu Medical University, Medicine, PR China
1993-1994 Visiting scientist, Institute of Clinical Biochemistry, University of Bonn, Germany

1994-1996 M.D Nanjing Medical University, Biochemistry, PR China
1996-1999 Ph.D Shanghai Medical University (presently, College of Medicine, Fudan University), Biochemistry, PR China
2000-2004 Postdoctoral fellow, University of Georgia, Department of Biochemistry and Molecular Biology, US
2004-present Research Assistant Professor, University of Georgia, Department of Biochemistry and Molecular Biology, US
Selected Publications:

Huabei Guo, Intaek Lee, Beau Bryan, and Michael Pierce. Deletion
of mouse embryo fibroblast N-acetylglucosaminyl-transferase V stimulates
a5b1 integrin expression mediated by the protein kinase C signaling pathway.
J.Biol.Chem., 2005; 280:8332-8342

Lee I, Guo HB, Kamar M, Abbott K, Troupe K, Lee JK, Alvarez-Manilla G,
Pierce M. N-acetylglucosaminyltranferase VB expression enhances beta1 integrin-
dependent PC12 neurite outgrowth on laminin and collagen. J Neurochem. 2006
May;97(4):947-56

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Karen Abbott
Position: Postdoctoral Associate
Lab: (706) 542-1701
Email: kabbott@uga.edu
Current project: Identification of glycoproteins that
have complex beta 1,6 branched glycan structures
as biomarkers for early detection of breast
carcinoma; Characterization of glycoprotein
acceptors and possible functions of GnT-Vb;
capture and characterization of
GPI-anchored glycans

Education and Honors:
1985-1988 B.S., Georgia College and State University, Milledgeville, GA, Magna Cum Laude
1989-1999 Senior Research Technician, Emory University, Atlanta, GA

1999-2004 Ph.D., Biochemistry, University of Georgia, Athens, GA
2005-2008 American Cancer Society Postdoctoral Research Fellowship, University of Georgia, CCRC
Selected Publications:

Abbott KL, Troupe K, Lee I, Pierce M. Integrin-dependent neuroblastoma
cell adhesion and migration on laminin is regulated by expression of two enzymes
in the O-mannosyl-linked glycosylation pathway, PomGnT1 and GnT-Vb. Exp
Cell Res.2006 312(15):2837-50.
Abbott KL, Lim J, Wells L, Pierce M. Identification of secreted proteins
from breast cancer cells that express a specific class of N-linked glycans
associated with invasiveness. 2006 Proteomics (in submission)

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Lori Hill
Position: Ph.D Student, Research Assistant
Lab: (706) 542-1701
Email: lorihill@uga.edu
Current project: Glycomics of Stem cells

Education and Honors:
1999-2003 B.S Furman University, Biology
2003-present Ph.D program, Department of Biochemistry and molecular biology, University of Georgia, Athens, GA
2004-present Ph.D program, Research Assistant in Pierce Lab
Selected Publications:

West AB, Zimprich A, Lockhart PJ, Farrer M, Singleton A, Holtom B, Lincoln
S, Hofer A, Hill L, Muller-Myhsok B, Wszolek ZK, Hardy J, Gasser T. Refinement
of the PARK3 locus on chromosome 2p13 and the analysis of 14 candidate
genes. Eur J Hum Genet. 2001 Sep;9(9):659-66.

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Matthew Randolph
Position: Research Assistant
Lab: (706) 542-1701
Email: mrandolp@uga.edu
Current project: work with different strains of mice
used by the Pierce laboratory and test novel
drugs as potential inhibitors for GnT-Va
glycosylation

Education and Honors:
1993-1996 Animal Science, University of Georgia
1996-2000 Doctorate of Veterinary Medicine, University of Georgia, College of Veterinary Medicine
2000-2005 Mixed Animal Medicine Practice
2005-present Research Assistant in Pierce lab
Selected Publications:

EF182744. Panicum virgatum retrotransposon centromeric Ty3/gypsy gag-pol
polyprotein gene, partial cds. gi|126632234|gb|EF182744.1|[126632234]
Hua-Bei Guo, Matthew Randolph, and J. Michael Pierce. Inhibition of a specific
N-glycosylation activity results in attenuation of breast carcinoma cell invasiveness-related
phentoypes. J Biological Chemistry. 2007 May 30.

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Nicole Warren
Position: Research Assistant
Lab: (706) 542-1701
Email: freshy@uga.edu
Current project: Cataloging glycoproteins from murine
stem cells to find stage-specific disease
markers; glycobiology of human renal
dipeptidase to find glycans involved
in proliferation of colorectal tumor
cells; biomarker glycosylation with
relation to prostate cancer

Education and Honors:
1999-2002 Associates of Arts, Northern Essex Community College
2002-2005 B.S. Biochemistry and Molecular Biology University of Georgia
2005-2007 Research Assistant in Pierce lab
2007-2011 A.T. Still University, College of Osteopathic Medicine
Selected Publications:

Alvarez-Manilla G, Warren NL, Abney T, Atwood J 3rd, Azadi P, York WS, Pierce M, Orlando R.
Tools for glycomics: relative quantitation of glycans by isotopic permethylation using 13CH3I.
Glycobiology. 2007 Mar 23; [Epub ahead of print]
Alvarez-Manilla G, Atwood J 3rd, Guo Y, Warren NL, Orlando R, Pierce M. Tools for glycoproteomic
analysis: size exclusion chromatography facilitates identification of tryptic glycopeptides with
N-linked glycosylation sites. J Proteome Res. 2006 Mar;5(3):701-8.

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Bradley Allen
Position: Undergraduate student
Email: jba@uga.edu
Graduation: Spring 2007
Post-graduation plans: Medical School

Barry Boriskie
Position: Undergraduate student
Email: bboriski@uga.edu
Graduation: Spring 2008
Post-graduation plans: Medical School

Lindsay Looft
Position: Undergraduate student
Email: lindsayl@uga.edu
Graduation: Spring 2008
Post-graduation plans: Medical School

Anant Mandawat
Position: Undergraduate student
Email: mandawat@uga.edu
Graduation: Spring 2008
Post-graduation plans: Medical School

Ifeoma Nwadei
Position: Undergraduate student
Email: ife409@uga.edu
Graduation: Spring 2007
Post-graduation plans: Medical School

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